The Bobcats said X-rays were negative, but Walker will not return.
The 6-foot Walker blocked a shot on one end of the floor and raced up court before stepping on Chris Bosh's foot as he attempted a layup. Walker immediately went down, pounding the court in obvious pain with his fist while holding his leg with the other hand.
The Bobcats were leading 64-57 at the time of the injury with Walker having six points and eight assists.
Walker has played in 190 straight games, the second-longest streak in franchise history.